Effects Of Ph On The Enzyme Activity

Aim: To investigate the effects of changing in PH on the activity of enzyme (trypsin). Hypothesis: Alternative hypothesis: As the PH level goes up, the enzyme activity of trypsin will speed up. The pursual represent is the prediction supporting my hypothesis. Introduction: Biology background (with theories) What ar enzymes? Enzymes act as biological catalysts; they make chemical reactions to head for the hills quicker and remains unchanged when the reaction finishes. accordingly enzymes ar used to speed up biological reactions in body. They dejection be working within cells and outside cells catalyzing metabolous reactions in human bodies. Enzymes are globular proteins; therefore they are alcohol-soluble and easily transported in blood. Enzyme has an active site in a specific see because of its tertiary structure. Enzyme works for book natural covering with substance of a specific define that fits in it and hurly gruff down the substance . Enzymes denature due to various factors. The temperature, PH level, and the parsimony of the substance influence enzyme activities. When these factors vary, enzymes may change in shape so it will not be able to fix to the specific substance anymore. What is trypsin? Trypsin is produced in the pancreas as typsinogen originally allowing metabolous restrain.

It is used widely in various biotechnological processes because it is very liberal to be purified. Method: Material: Trypsin Casein Water dishwashing tub (to keep the temperature perpetual) Colorimeter (to prize the rate of enz yme activity) Thermometer seek Tubes (to! contain the casein and the buffer solution) Stop watch (to control and measure the time) Distilled water (to mix with trypsin to produce the buffer solution) mental screen outing supply rack (to prevent the tubes rolling and smashing) Protocol: redact 2.5 cm³ of 5% with reconstituted casein in 6 of the test tubes (Tube A) derriere the test tubes into the water bath at a constant temperature for 5 minutes....If you want to get a proficient essay, influence it on our website: OrderCustomPaper.com

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